Investigation of the effect of trisodium-citrate on blood coagulation by viscometric approach

Abstract Blood coagulation is a process, which initiated by certain physico-chemical effects. This process results in change the blood from sol state, that well suited for further processing, to gel state. 13 clotting factors take part cascade system of coagulation. Trisodium-citrate affects factor IV, calcium, and prevents texture. The effect different concentrations trisodium-citrate (0, 0.48, 2.4, 4.8, 9.6, 14.4, 19.2, 24 w/w%) on texture investigated. Porcine was collected 20 cm 3 test tubes slaughterhouse directly before addition stored one day under refrigerated conditions. samples without coagulated with high (4–5 g) became solid as because protein salting-out. viscosity successfully treated shear stress were measured rotational viscometer (Physica MCR 51, Anton-Paar) concentric cylinders Couette type method. flow behavior all could be described Herschel-Bulkley model. yield point, consistency index power law index, are determined equation model, showed lower content “better” sample most similar Newtonian fluid. trend-likes, but significant differences may expected case higher amount. point sample, contained 14.4 w/w% trisodium-citrate, 37.3% less than containing 0.48% g 20.5% trisodium-citrate. Thanks these cheaper concentration drying porcine fractions available no surplus water added blood.